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pypq266a  (Addgene inc)


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    Structured Review

    Addgene inc pypq266a
    Pypq266a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pypq266a/Flag-NHR2%2BR+pcDNA3%2E1+(Plasmid+%2317317)/pmc08882796-195-3-4
    Average 92 stars, based on 2 article reviews
    pypq266a - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Generated:

    Article Title: CRISPR‐BETS: a base‐editing design tool for generating stop codons
    Article Snippet: The nCas9(D10A)‐NG‐PmCDA1 vector, pYPQ266A (Addgene # 173176), was prepared using NEBuilder® HiFi DNA Assembly Master Mix kit (NEB, catalog #E2621*) from PCR‐amplified fragments of vectors pYPQ266 (Ren et al ., ) and a zCas9‐NG fragment. .. The T‐DNA vectors were generated in three‐way Gateway reactions with one attL1‐attR5 base editor entry clone such as pYPQ266 (Addgene #164713), pYPQ266E (Addgene #161521) or pYPQ266A (Addgene # 173176), one attL5‐attL2 pYPQ141B vector containing one cloned gRNA and the attR1‐attR2 destination vector pCGS710, according to our previously established protocol (Sretenovic et al ., ). ..

    Clone Assay:

    Article Title: CRISPR‐BETS: a base‐editing design tool for generating stop codons
    Article Snippet: The nCas9(D10A)‐NG‐PmCDA1 vector, pYPQ266A (Addgene # 173176), was prepared using NEBuilder® HiFi DNA Assembly Master Mix kit (NEB, catalog #E2621*) from PCR‐amplified fragments of vectors pYPQ266 (Ren et al ., ) and a zCas9‐NG fragment. .. The T‐DNA vectors were generated in three‐way Gateway reactions with one attL1‐attR5 base editor entry clone such as pYPQ266 (Addgene #164713), pYPQ266E (Addgene #161521) or pYPQ266A (Addgene # 173176), one attL5‐attL2 pYPQ141B vector containing one cloned gRNA and the attR1‐attR2 destination vector pCGS710, according to our previously established protocol (Sretenovic et al ., ). ..

    Polymerase Chain Reaction:

    Article Title: CRISPR‐BETS: a base‐editing design tool for generating stop codons
    Article Snippet: Similarly, paired oligos (Table ) for each target site were generated, annealed and inserted into pYPQ141B (Addgene #69291; Lowder et al ., ). .. The nCas9(D10A)‐NG‐PmCDA1 vector, pYPQ266A (Addgene # 173176), was prepared using NEBuilder® HiFi DNA Assembly Master Mix kit (NEB, catalog #E2621*) from PCR‐amplified fragments of vectors pYPQ266 (Ren et al ., ) and a zCas9‐NG fragment. .. The T‐DNA vectors were generated in three‐way Gateway reactions with one attL1‐attR5 base editor entry clone such as pYPQ266 (Addgene #164713), pYPQ266E (Addgene #161521) or pYPQ266A (Addgene # 173176), one attL5‐attL2 pYPQ141B vector containing one cloned gRNA and the attR1‐attR2 destination vector pCGS710, according to our previously established protocol (Sretenovic et al ., ).



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